Mueller Hinton agar is recommended for use in the antibiotic disk diffusion method by both the European Committee on Antibiotic Sensitivity Testing (EUCAST) and the Clinical Laboratory Standards Institute (CLSI). The medium contains low levels of divalent metal cations, such as calcium and magnesium, to minimise any interference with certain antibiotic classes e.g. aminoglycosides. Starch is also present to absorb any toxic metabolites that may be formed during growth. The medium is low in thymine and thymidine content and is therefore suitable for use in testing sulphonamides and trimethoprim without the addition of blood.
- Storage temperature: 2 to 8°C
- Appearance: firm gel
- Colour: straw
- Recommended incubation: aerobically at 35°C ± 1°C for 16 to 20 hours
- pH: 7.3 ± 0.2
- Plate size: 120mm
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